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復(fù)合酶法改善大豆分離蛋白乳化性的試驗
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    運用配料試驗設(shè)計解決了多酶復(fù)配的比例優(yōu)化問題。采用米曲霉蛋白酶、木瓜蛋白酶、胰蛋白酶對大豆分離蛋白進行水解,建立復(fù)合酶配合比例與乳化性之間的數(shù)學模型。確定最佳比例為:米曲霉蛋白酶11.79%、胰蛋白酶32.93%、木瓜蛋白酶55.28%。最佳水解條件為:復(fù)合酶溫度40℃、底物質(zhì)量分數(shù)9%、酶添加量3%、pH值7.5、水解時間3h,乳化能力比原料提高了

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    50.86%。 An experimental method based on controlled mixture ratio of different enzymes in order to improve the emulsifying properties of soybean protein isolate was proposed. The model between the emulsifying properties of partial hydrolysis soybean protein and mixture ratio with aspergillus oryzae proteinase, papain and trypsin was established. The result of enzymes proportion was that aspergillus oryzae protease was 11.79%, papain was 32.93%, trypsin was 55.28%. The optimum hydrolysis condition was obtained by adding 3% of the multiple enzymes/substrate mass ratio to soybean protein isolate by way of dissolving at 9% in water and incubating 3?h. at 40℃ for pH7.5. The emulsifying capacity of hydrolyzing soybean protein with composite enzymes was increased up to 50.86% compared to the control sample.

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徐紅華,劉欣,溫其標.復(fù)合酶法改善大豆分離蛋白乳化性的試驗[J].農(nóng)業(yè)機械學報,2008,39(2):103-106.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(2):103-106.

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