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基于紫外指紋圖譜技術(shù)的食醋品種檢測方
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of Vinegar Detection Based on Ultraviolet Fingerprint Technology
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    摘要:

    以不同食醋為研究對象,采用旋轉(zhuǎn)蒸發(fā)、紫外光譜掃描等手段研究了波長范圍、蒸發(fā)液稀釋比例、蒸發(fā)溫度和參比液質(zhì)量濃度等對食醋紫外吸收曲線的影響,確定了食醋紫外指紋圖譜檢測方法的試驗條件:掃描波長范圍245~330nm,蒸發(fā)液稀釋比例1∶6,蒸發(fā)溫度45℃,冰醋酸參比液質(zhì)量濃度45g/L。以曲線間的相似度為指標驗證了方法的重現(xiàn)性、穩(wěn)定性和差異性,結(jié)果表明:各個食醋樣品3次重復(fù)測定的指紋圖譜間相似度均大于0.90,同一樣品圖譜的重現(xiàn)性良好;各個食醋樣品在不同存放期的紫外指紋圖譜間相似度均大于0.90,同一樣品圖譜的穩(wěn)定性良好;各個不同食醋樣品的紫外指紋圖譜之間的相似度均小于0.90,不同樣品圖譜的差異性良好。

    Abstract:

    In order to find a method of vinegar detection based on ultraviolet fingerprint technology, different vinegars were used as materials, evaporation and ultraviolet spectra were studied to investigate how the factors of wavelength, dilution ratio of the liquid, evaporation temperature and mass concentration of reference affect ultraviolet vinegar curves. Finally the experiment conditions of the method were determined: wavelength at 245~330nm, quantity dilution ratio of the liquid at 1∶6,evaporation temperature at 45℃, mass concentration of reference at 45g/L. Similarity was used as the indicator to evaluate the ultraviolet curves’ feature of reproducibility, stability and difference. Three replicates of each measurement the result show that the similarity among vinegar samples fingerprints was more than 0.90, the similarity of the fingerprint of each vinegar samples in different depositing time was more than 0.90, the similarity of different fingerprint of different vinegar samples was less than 0.90. The method with excellent repeativeness, stability and differentiae.

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解華東,布麗君,李志西.基于紫外指紋圖譜技術(shù)的食醋品種檢測方[J].農(nóng)業(yè)機械學(xué)報,2009,40(4):133-138. of Vinegar Detection Based on Ultraviolet Fingerprint Technology[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(4):133-138.

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