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酶解法提高松針黃酮抗氧化性能試
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on Enzymatic Hydrolysis to Improve in Vitro Anti-oxidative Activity of Pine Needle Flavonoids
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    研究了松針總黃酮(PNF）的抗氧化性能和酶法修飾提高PNF抗氧化性能的條件。結(jié)果發(fā)現(xiàn)，PNF的體外抗氧化性能很強(qiáng)，DPPH自由基清除效果與抗壞血酸相似，羥基自由基、超氧陰離子清除率分別約為抗壞血酸清除率的4倍、2倍，F(xiàn)e3+還原能力的EC50約為抗壞血酸EC50的1/2.8。用β-葡萄糖苷酶對(duì)PNF進(jìn)行酶解修飾，其最優(yōu)工藝參數(shù)為：酶解溫度40℃，酶添加量1/1000，底物質(zhì)量濃度0.6g/L，酶解時(shí)間5h。酶解后，PNF的抗氧化性能明顯提高。

    Abstract:

    The in Vitro anti-oxidative activity of pine needle flavonoids（PNF）and enzymatic hydrolysis were investigated. It is found that the DPPH radical scavenging activity of PNF is similar to that of ascorbic acid. The hydroxyl radical and super-oxide radical scavenging activity of PNF are 4 times and 2 times as that of ascorbic acid, respectively. As for reducing power, EC50 ratio of PNF to ascorbic acid is only 1/2.8. β-glucosidase is used to hydrolyze PNF. The optimal hydrolyzing conditions are 40℃ of reaction temperature，1/1000 of enzyme addition，0.6g/L of substrate concentration, and 5h of reaction time. The anti-oxidative activity of enzymatic hydrolyzed PNF is increased obviously. 

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殷涌光,閆琳娜,于慶宇.酶解法提高松針黃酮抗氧化性能試[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2009,40(6):129-132. on Enzymatic Hydrolysis to Improve in Vitro Anti-oxidative Activity of Pine Needle Flavonoids[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(6):129-132.

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