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大豆蛋白限制性酶解對(duì)乳化性質(zhì)和吸油性的影響?yīng)?/div>
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of Soybean Proteins and Modifications in Emulsifying Property and Oil Absorption Capacity
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    摘要:

    利用中性蛋白酶和胰蛋白酶對(duì)大豆?jié)饪s蛋白、分離蛋白進(jìn)行限制性酶解處理,以SDS-PAGE分析評(píng)價(jià)酶解產(chǎn)品的蛋白質(zhì)降解情況。評(píng)價(jià)水解度為1%、2%的8個(gè)酶解產(chǎn)品的乳化活性指數(shù)、乳化穩(wěn)定性、吸油率,考察酶解產(chǎn)品的酶解模式與乳化性質(zhì)、吸油性變化的關(guān)系。結(jié)果表明,酶解產(chǎn)品的乳化性質(zhì)、吸油性變化與所使用的酶或水解度有關(guān)。大豆?jié)饪s蛋白的限制性酶解可以提高產(chǎn)品的乳化性質(zhì)和吸油性,水解度為1%的胰蛋白酶酶解產(chǎn)品具有最好的乳化性質(zhì)和吸油性。大豆分離蛋白的限制性酶解也可以提高產(chǎn)品的乳化活性指數(shù),但降低了其吸油性;水解度為1%的胰蛋白酶酶解產(chǎn)品也具有最好的乳化性質(zhì)。

    Abstract:

    Soybean protein concentrate and soybean protein isolate were both limited hydrolysed with neutrase and trypsin respectively. SDS-PAGE was employed to analyse protein degradation in the hydrolysed soybean protein products. The relationship between hydrolysis pattern and the functional properties of the hydrolysed soybean protein products, such as emulsion property and oil absorption, were investigated. The emulsifying activity index, emulsion stability and oil absorption capacity of eight hydrolysed soybean protein products with the degree of hydrolysis (DH) of 1% or 2% were detected. The experimental data indicate that the emulsifying property and oil absorption capacity correlate with the protease used or the DH. For soybean protein concentrate, hydrolysis with neutrase or trypsin improves its emulsifying property and oil absorption capacity, and hydrolysis with trypsin to the DH of 1% gains the optimal emulsifying property and oil absorption capacity. At the same time, hydrolysis with neutrase or trypsin improves the emulsifying activity index of soybean protein isolate, but reduces the oil absorption capacity, and hydrolysed soybean protein isolate with trypsin to a DH of 1% has the optimal emulsion property.

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趙新淮,侯瑤.大豆蛋白限制性酶解對(duì)乳化性質(zhì)和吸油性的影響?yīng)琜J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2009,40(12):159-163. of Soybean Proteins and Modifications in Emulsifying Property and Oil Absorption Capacity[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(12):159-163.

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