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In order to help the farmers to obtain the agricultural knowledge to prevent and control the pests and diseases as well as to carry on the remote diagnosis by telephone, a call center system was presented for agricultural knowledge based on the remote diagnosis of pests and diseases. Based on the union of the call center technology and the traditional expert system, this system adopted the implementation plan of call center IVR flow transferring the expert system knowledge base automatically, and coordinated the call center CTI server the traditional database server and Web server to provide the function of carried on the remote diagnosis of agricultural pests and diseases with the aid of the telephone network; the system also provided the functions of knowledge browsing, case gaining and expert consultation, and could satisfy the farmers needs to gain the agricultural knowledge as well as to carry on the remote diagnosis.