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南瓜多糖大孔吸附樹脂純化工藝
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Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin
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    摘要:

    為提高南瓜多糖的純度，篩選適宜南瓜多糖純化的大孔樹脂，確定純化工藝參數(shù)，選用9種大孔樹脂材料，采用靜態(tài)與動態(tài)吸附-解吸方法對南瓜多糖進(jìn)行純化，以吸附量與解吸率為考察指標(biāo)對樹脂進(jìn)行選擇與工藝研究。結(jié)果表明：D101-Ⅰ型大孔樹脂更適宜作為南瓜多糖純化的樹脂；其純化最佳工藝條件為：上柱液質(zhì)量濃度4.79mg/mL，上柱液體積3.2BV，上柱流速3BV/h，洗脫液乙醇體積分?jǐn)?shù)25%，洗脫速度4BV/h，洗脫液體積3.6BV；上述純化工藝使南瓜多糖純度從

    Abstract:

    25.68%提高到61.39%。In order to select suitable macroporous resin, to improve the purity of pumpkin polysaccharide and to determine purification process parameters, 9 kinds of macroporous resin were chosen to carry out static and dynamic adsorption-desorption experiments. With the indexes of adsorption capacity and elution rate, the suitable macroporous resin was selected and process parameters were confirmed. The results show that D101-I resin has the best performance of adsorption and desorption effects for purification of pumpkin polysaccharide, the optimum technology parameters are sample concentration of 4.79mg/mL, sample volume of 3.2BV, flow rate of 3BV/h, 25% ethanol for elution, elution velocity of 4BV/h, elution volume of 3.6BV. The purity of pumpkin polysaccharide increases from 25.68% to 61.39% by using the above technology. 

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王麗波,趙玉,徐雅琴.南瓜多糖大孔吸附樹脂純化工藝[J].農(nóng)業(yè)機(jī)械學(xué)報,2010,41(9):138-142.Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(9):138-142.

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