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超聲波預(yù)處理對(duì)固定化纖維素酶活性的影響
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江蘇省自然科學(xué)基金資助項(xiàng)目（BK2008241）、江蘇大學(xué)高級(jí)專業(yè)人才科研啟動(dòng)基金資助項(xiàng)目（08JDG003）、中國博士后科學(xué)基金資助項(xiàng)目（20090461072）和江蘇省博士后科學(xué)基金資助項(xiàng)目（0902108C）


Effect on Activity of Immobilized Cellulase by Ultrasound Assisted Pretreatment
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    摘要:

    采用超聲波預(yù)處理固定化纖維素酶，通過單因素試驗(yàn)和響應(yīng)面法探討了超聲波預(yù)處理?xiàng)l件（超聲時(shí)間、超聲頻率、超聲功率）以及預(yù)處理后的酶解溫度和CMC-Na緩沖液pH值對(duì)固定化纖維素酶活性的影響，建立并分析了各因子與酶活相對(duì)關(guān)系的數(shù)學(xué)模型，優(yōu)化得到的最佳條件為：酶解溫度58.73℃、CMC-Na緩沖液pH值3.0、超聲時(shí)間16.88min、超聲頻率22.33kHz、超聲功率26.77W，在此條件下，固定化纖維素酶活性與未加超聲波預(yù)處理相比較提高了9.75%。

    Abstract:

    Immobilized cellulase was pretreated by ultrasound. Based on the single factor and response surface methodology, the effects of ultrasonic parameters, such as ultrasonic time, ultrasonic frequency and ultrasonic power, as well as enzymolytic temperature and pH value of CMC-Na buffer solution after pretreated were investigated. A mathematical model was established and analyzed to describe the relationships between the studied factors and enzyme activity. The optimum treatment parameters were as follows: enzymolytic temperature of 58.73℃, pH value of CMC-Na buffer solution of 3.0, ultrasonic time of 16.88min, ultrasonic frequency of 22.33kHz and ultrasonic power of 26.77W. Under the optimum conditions, enzyme activity of immobilized cellulose increased by 9.75%.

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王振斌,張杰,王世清,馬海樂,王倩倩,陳曉寅.超聲波預(yù)處理對(duì)固定化纖維素酶活性的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2011,42(3):150-155. Wang Zhenbin, Zhang Jie, Wang Shiqing, Ma Haile, Wang Qianqian, Chen Xiaoyin. Effect on Activity of Immobilized Cellulase by Ultrasound Assisted Pretreatment[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(3):150-155.

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