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The electrical conductivity method for determination of acid value in edible oil was developed based on the electrical conductivity change value of alkaline solution during reaction, and the effects on determination of the varying condition, as concentration of the alkaline solution, types of the oil samples, temperature and the solid-liquid ratio were explored. The method was validated by Chinese national standard methods. The results showed the experimental conditions had no significant effect on the determination. When the solid-liquid ratio was 1∶10, the calibration was y=0.0024x-0.1571 (where x is the value of the conductivity change, y is acid values), R2=0.9984. The linear correlativity of the determination of the acid value between two methods was very well, the relative deviation of blind samples experiment’s data were less than 10%.