亚洲一区欧美在线,日韩欧美视频免费观看,色戒的三场床戏分别是在几段,欧美日韩国产在线人成

超聲聚焦微噴制備脂質(zhì)體微陣列研究
作者:
作者單位:

作者簡介:

通訊作者:

中圖分類號:

基金項(xiàng)目:

國家自然科學(xué)基金資助項(xiàng)目(51005139);山東省高等學(xué)??萍加?jì)劃資助項(xiàng)目(J10LD03)


Fabrication of the Liposome Microarrays Based on the Ultrasonic Focusing Microjet System
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 圖/表
  • |
  • 訪問統(tǒng)計(jì)
  • |
  • 參考文獻(xiàn)
  • |
  • 相似文獻(xiàn)
  • |
  • 引證文獻(xiàn)
  • |
  • 資源附件
  • |
  • 文章評論
    摘要:

    設(shè)計(jì)了基于超聲換能器和球面聲透鏡的超聲聚焦微噴頭及向下隔離噴射機(jī)構(gòu),實(shí)現(xiàn)了無噴嘴、無堵塞、非接觸、低消耗、高精度的微陣列制備。為驗(yàn)證其生物相容性,以載有F0F1—ATP酶的分子馬達(dá)脂質(zhì)體為對象,制備出了形狀規(guī)則、定位準(zhǔn)確的脂質(zhì)體微陣列,并對其ATP水解活性和合成活性進(jìn)行了檢測。實(shí)驗(yàn)結(jié)果表明,所制備的微陣列生物芯片在相關(guān)光化學(xué)刺激下具有良好的ATP水解與合成活性,從而證實(shí)該方法在微陣列生物芯片制備過程中能夠較好地保持樣品的生物活性。

    Abstract:

    Focusing on the problems existing in the biochip microarray manufacturing methods such as low maintenance in bioactivity, microjet head blockage and high consumption in biological reagent and so on, the ultrasonic transducer and spherical acoustic lens based on ultrasonic focusing microjet head as well as the downward isolated jetting mechanism was designed. Based on this, no nozzle, non blockage, non contact, low consumption and high accuracy was gained in the microarray manufacture. To verify the biocompatibility of the microjet system, the F0F1—ATP molecular-motor assembled liposome microarrays with regular shape and accurate location was prepared. Furthermore, the ATP hydrolysis activity and the ATP synthesis activity of the liposome microarrays were tested respectively. Experimental results indicated that the liposome microarrays fabricated this way showed favorable ATP hydrolysis activity and synthesis activity, which confirmed that the ultrasonic focusing microjet method has favorable biocompatibility in manufacturing biological microarrays. The investigation elaborated here explored a new manufacturing approach for biological microarrays.

    參考文獻(xiàn)
    相似文獻(xiàn)
    引證文獻(xiàn)
引用本文

韓鑫,張波,李筍,張德遠(yuǎn).超聲聚焦微噴制備脂質(zhì)體微陣列研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2011,42(11):231-234. Han Xin, Zhang Bo, Li Sun, Zhang Deyuan. Fabrication of the Liposome Microarrays Based on the Ultrasonic Focusing Microjet System[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(11):231-234.

復(fù)制
分享
文章指標(biāo)
  • 點(diǎn)擊次數(shù):
  • 下載次數(shù):
  • HTML閱讀次數(shù):
  • 引用次數(shù):
歷史
  • 收稿日期:
  • 最后修改日期:
  • 錄用日期:
  • 在線發(fā)布日期:
  • 出版日期: