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條斑紫菜ACEI抑制肽的超濾分離與離子交換純化
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中國博士后科學基金資助項目（20100471386）、江蘇省博士后科學基金資助項目（0902029C）、浙江省自然科學基金資助項目（Y3110025）、江蘇高校優(yōu)勢學科建設(shè)工程資助項目、南通市產(chǎn)學研聯(lián)合創(chuàng)新科技計劃資助項目（B2010008）和江蘇大學高級人才科研啟動基金資助項目（08JDG032）


Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein
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    摘要:

    對條斑紫菜ACEI抑制肽進行了超濾分離和離子交換純化的試驗研究。先后采用截留分子量分別為10 kDa和3 kDa的超濾膜對條斑紫菜ACEI抑制肽超濾，分子量小于3 kDa的組分對ACE活性抑制最高，多肽的IC50值從超濾前的0.844 mg/mL下降到0.523 mg/mL。南開D61陽離子樹脂對分子量小于3 kDa的ACEI抑制肽組分吸附率最大，用不同濃度氨水階梯洗脫，得到第2個洗脫峰的蛋白回收率和活性回收率最高，分別為45.2%和61.5%，其IC50值最低，為0.127 mg/mL，比純化前下降了75.7%。

    Abstract:

    Angiotensin I-converting enzyme (ACE) inhibitory peptides from digested Porphyra yezoensis protein were purified with ultrafiltration and ionic exchange. The hydrolysate of Porphyra yezoensis protein was ultrafiltered by 10 kDa and 3 kDa membrane in sequence. It was found that the IC50 value of Porphyra yezoensis peptides decreased from original 0.844 mg/mL to 0.523 mg/mL. And Nakai D61 cation resin was selected according to its largest absorption in the ionic exchange static absorption test. The results showed that the second elution peak had the highest protein recovery, ACE inhibitory activity recovery and the lowest IC50 value, which were 45.2%, 61.5% and 0.127 mg/mL, respectively. The sample’s IC50 value decreased by 75.7% after the ionic exchange purification.

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何榮海,馬海樂,余筱潔.條斑紫菜ACEI抑制肽的超濾分離與離子交換純化[J].農(nóng)業(yè)機械學報,2012,43(2):146-150,204. He Ronghai,Ma Haile, Yu Xiaojie. Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(2):146-150,204.

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  • 在線發(fā)布日期: 2012-02-17
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