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油茶籽糖蛋白提取工藝優(yōu)化及抗氧化性
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江蘇省高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目(PAPD)


Extraction Optimization and Antioxidant Activity of Glycoprotein from Camellia oleifera Seed
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    摘要:

    為探討油茶籽糖蛋白的最優(yōu)提取工藝及抗氧化活性,在單因素試驗(yàn)基礎(chǔ)上采用響應(yīng)面法對(duì)提取油茶糖蛋白的關(guān)鍵參數(shù)進(jìn)行了優(yōu)化。同時(shí)以維生素C作為對(duì)照,采用4種不同方法考察了油茶籽糖蛋白的抗氧化性能。結(jié)果表明,油茶籽糖蛋白的最佳提取工藝條件為:浸提時(shí)間8.81h、提取鹽濃度0.12mol/L、pH值8.77和液料比11.62mL/g,在此條件下蛋白得率為8.76%,糖得率為10.14%??寡趸囼?yàn)表明,油茶籽糖蛋白能顯著清除DPPH、羥基自由基和超氧陰離子自由基,具備一定的還原能力。

    Abstract:

    The optimum extraction technology and antioxidant activity of glycoprotein from Camellia oleifera seed were performed for future development and utilization of the Camellia oleifera. On the base of singlefactor experiment, the optimum parameters of extraction for glycoprotein from Camellia oleifera seed were determined by response surface methodology. The antioxidant activity of glycoprotein was examined by using various antioxidant assays with vitamin C co-assayed as positive control. As a result, the optimized condition of glycoprotein extraction was as follows: extraction time of 8.81h, salinity of 0.12mol/L, pH value of 8.77, ratio of liquid to solid of 11.62mL/g. At the optimum conditions, the yield of protein reached to 8.76%, and the yield of saccharide reached to 10.14%. The results of above antioxidant tests demonstrated that the glycoprotein from Camellia oleifera seed has certain antioxidant activities.

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李婷婷,張暉,吳彩娥,范龔建.油茶籽糖蛋白提取工藝優(yōu)化及抗氧化性[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2012,43(4):148-155. Li Tingting, Zhang Hui, Wu Caie, Fan Gongjian. Extraction Optimization and Antioxidant Activity of Glycoprotein from Camellia oleifera Seed[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(4):148-155.

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  • 在線發(fā)布日期: 2012-04-18
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