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In order to investigate the feasibility of sample-sample 2-D correlation near infrared diffuse reflectance spectroscopy method for different protein feeds rapid identification，52 fish meal samples and 60 soybean meal samples were collected，including 74 calibration samples and 38 independent validation samples. Samples were measured by spectrometer. Calibration model was established by sample-sample 2-D correlation spectroscopy method with the pretreatment of 3-point first derivative. This method performed 100% of correctly classified samples. Compared with partial least squares discriminant analysis method, both of them can correctly identify the fish meal and soybean meal. But the sample-sample 2-D correlation spectroscopy method is fast for calculation.