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In order to make sure the distribution of Alicyclobacillus from apple libraries in Shaanxi province, the Alicyclobacillus in apple and air were isolated and purified by using acidified AAM medium. The cell morphology, colony morphology, growing conditions, cultural characteristics and physiological characteristics were compared with the reference strain A.acidoterrestris DSM3922T. A phylogenetic tree based on 16S rDNA sequence analysis was constructed. The genetic location of eight isolated strains was clear. The isolated strain LC-4 was basically consistent with the reference stain Alicyclobacillus acidoterrestris DSM3922 T in morphology and physiological characteristics. By 16S rDNA sequence analysis, isolated strain LC-4 shared a homology of 99% with the reference strain, so it belonged to A.acidoterrestris and the other seven strains belonged to Bacillus ginsengihuimi. The results suggested Alicyclobacillus was distributed in orchard soil, which might pose a threat to the quality of fruit juice, and needed to be controlled.