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In order to detect the wheat biomass non-destructively and fast, a system based on LabVIEW was developed according to the mechanical property of wheat stalk. Real-time acquisition, display and storage of the data was finished by detection system based on LabVIEW, with mechanical sensor as measuring element and collecting resilience signal produced when the stalk was pushed by the rod with high-speed acquisition module DAM—3056AH of ART-TECH. The detection system was tested in the wheat field to examine its property. The results showed that the resilience of the wheat stalks between different varieties were clearly different. The resilience of the wheat stalk had close relationship with biomass (fresh weight), of which the R2 was achieved 0.7127. It was indicated that the detection of wheat biomass based on mechanics was feasible.