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In order to judge the degree of plant disease stress effectively and determine disease situation, according to plant acoustic emission phenomenon, cylindrical and spherical non-conformal acoustic holography experiment model was established based on the distributed source boundary point method near-field acoustic holography technology. Single sound source and double sound sources were simulated respectively. The parameters, such as the holographic cylindrical radius, the number of measuring points on the holographic face, the number of nodes on the reconstructed spherical surface, the distance from fictional special point sound source to nodes on the reconstructed spherical surface and so on should be adjusted to obtain the practicable experimental parameters. The simulation results showed that the method could identify and locate the position of the acoustic emission source accurately. According to the experimental parameters, the signal acquisition acoustic holography experiment system was established based on DSP, signal was analyzed and processed based on LabView software platform.