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鉬脅迫對(duì)煙草光合熒光參數(shù)和葉綠體超微結(jié)構(gòu)的影響
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國(guó)家自然科學(xué)基金資助項(xiàng)目(41301539)、安徽省教育廳高校重點(diǎn)基金資助項(xiàng)目(KJ2013Z085)和貴州省煙草公司重點(diǎn)科技項(xiàng)目(201105)


Effects of Photosynthetic Fluorescence Parameters and Chloroplast Ultrastructure of Tobacco under Molybdenum Stress
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    摘要:

    為探明煙草響應(yīng)鉬脅迫光合生理機(jī)制,研究了缺鉬脅迫對(duì)煙草光合氣體交換、葉綠素?zé)晒鈪?shù)和葉綠體超微結(jié)構(gòu)的影響。結(jié)果表明:缺鉬培養(yǎng)10d后,煙苗光合色素含量和凈光合速率較對(duì)照有所降低,胞間二氧化碳濃度、氣孔導(dǎo)度和蒸騰速率提高,光系統(tǒng)初始熒光和非光化學(xué)猝滅系數(shù)提高,光合電子傳遞速率和最大光化學(xué)效率降低,但差異均不顯著;煙苗的葉綠體基類片層和基質(zhì)片層排列松散,淀粉粒數(shù)量少。培養(yǎng)20d時(shí),缺鉬和對(duì)照煙苗的多數(shù)指標(biāo)差異顯著。培養(yǎng)30d時(shí),除蒸騰速率外,缺鉬和對(duì)照煙苗的其它指標(biāo)差異均顯著,葉綠體片層結(jié)構(gòu)受損嚴(yán)重,淀粉粒發(fā)育差。缺鉬脅迫損傷了葉綠體超微結(jié)構(gòu),抑制了原初光能轉(zhuǎn)化效率,降低了光合能力。且隨脅迫時(shí)間延長(zhǎng),缺素表現(xiàn)明顯。

    Abstract:

    With the aim to investigate the physiological mechanism of tobacco response to molybdenum stress, the effect of photosynthetic pigment, gas exchange parameters,chlorophyll fluorescence parameters and chloroplast ultrastructure of tobacco were studied under molybdenum deficiency stress by the hydroponic experiment. The results showed that, compared with the control group, the photosynthetic pigment contents and net photosynthetic rate of tobacco leaves cultivated about 10d decreased, while intercellular CO2 concentration, stomatal conductance and transpiration rate increased. And the initial fluorescence and non-photochemical quenching coefficient (NPQ) of PS II increased, while photosynthetic electron transport rate (ETR) and the maximal photochemical efficiency decreased,but the differences between two treatments were not significant. The grana layer of the chloroplast ultrastructure under molybdenum deficiency stress expanded to deformation and loose,the number of amyloid was less and it grew worse. The most indicators between molybdenum deficiency and control tobacco cultivated about 20d were significant. In addition to Tr, other indexes between molybdenum deficiency and control tobacco cultivated about 30d were significant, and the chloroplast under the molybdenum deficiency stress was damaged seriously. Therefore,under the molybdenum deficiency stress,the chloroplast ultrastructure grows worse, the activity of PS II is inhibited, and the Pn decreases. With the extension of molybdenum stress time, the deficiency reaction is more obvious.

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武麗,李章海,葉文玲,黃義德.鉬脅迫對(duì)煙草光合熒光參數(shù)和葉綠體超微結(jié)構(gòu)的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2014,45(8):262-268. Wu Li, Li Zhanghai, Ye Wenling, Huang Yide. Effects of Photosynthetic Fluorescence Parameters and Chloroplast Ultrastructure of Tobacco under Molybdenum Stress[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(8):262-268.

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  • 收稿日期:2014-03-24
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  • 在線發(fā)布日期: 2014-08-10
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