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能源草酶解光合生物制氫實(shí)驗(yàn)研究
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國家自然科學(xué)基金資助項(xiàng)目(51376056)、國家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)資助項(xiàng)目(2012AA051502)和教育部博士點(diǎn)基金優(yōu)先領(lǐng)域資助項(xiàng)目(20134105130001)


Photo-hydrogen Production of Energy Grasses Pretreated by Enzymatic Hydrolysis
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    摘要:

    利用王草、象草、柳枝稷、紫花苜蓿這4種常見能源草的纖維素酶酶解液作為產(chǎn)氫底物,對其光合生物制氫性能進(jìn)行了實(shí)驗(yàn)研究,以累積產(chǎn)氫量和產(chǎn)氫速率為考察指標(biāo),對比了不同類型能源草的產(chǎn)氫能力,并利用修正的Gompertz方程對產(chǎn)氫過程進(jìn)行回歸分析,驗(yàn)證了能源草作為光合制氫原料的技術(shù)可行性。結(jié)果表明,當(dāng)產(chǎn)氫工藝條件為光合細(xì)菌接種量30%、溫度30℃、光照度2000lx、發(fā)酵時(shí)間120h時(shí),紫花苜蓿產(chǎn)氫性能最好,王草次之,而象草和柳枝稷的產(chǎn)氫性能較差。王草、象草、柳枝稷和紫花苜蓿的累積產(chǎn)氫量分別為75.3、27.2、26.1和81.6mL,最大產(chǎn)氫速率分別為7.83、3.5、4.33、14.75mL/(h?L)。

    Abstract:

    This paper mainly studied the photo-hydrogen production capability of using enzymatic hydrolysate to four different energy grasses, king grass, witchgrass, grassiness and alfalfa, as substrates. The cumulative hydrogen production and hydrogen production rate were taken as the indexes to contrast photo-hydrogen production capability of different energy grasses. Then modified Gompertz equation was used to perform regression analysis of hydrogen production process, and the technical feasibility using energy grasses as raw materials for photo-hydrogen production was verified. The results showed that among these four energy grasses, the hydrogen producing capability of alfalfa was the best followed closely by king grass, and the hydrogen producing capabilities of witchgrass and grassiness were feeblish, in the circumstances of 30% inoculum size, 30℃, 2000lx illuminance, 120h of fermentation time. The cumulative hydrogen production of king grass, witchgrass, grassiness and alfalfa was respectively 75.3, 27.2, 26.1, and 81.6mL. The maximal hydrogen production rate was 7.83, 3.5, 4.33, and 14.75mL/(h?L) respectively.

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張全國,張丙學(xué),蔣丹萍,李亞猛,荊艷艷,路朝陽.能源草酶解光合生物制氫實(shí)驗(yàn)研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2014,45(12):224-228,261. Zhang Quanguo, Zhang Bingxue, Jiang Danping, Li Yameng, Jing Yanyan, Lu Chaoyang. Photo-hydrogen Production of Energy Grasses Pretreated by Enzymatic Hydrolysis[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(12):224-228,261.

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  • 收稿日期:2014-07-30
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  • 在線發(fā)布日期: 2014-12-10
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