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Based on researches of light-temperature production potential, availability and regional planning of rice in Hubei province using GIS method, this paper reported the establishment of GIS based database by combination of mechanism method and experience approach, with the support of data of sub counties’ (cities, districts) rice actual production, meteorological data, terrain and etc. in Hubei province between years 1993—2012. The potential productivities of light, temperature, climate, arable land, yield-increasing potential and potential increase space were calculated by considering rules of production and development of middle-season rice. The yield potential was divided into five districts and the charts were drawn respectively. Natural resources and utilization status in different middle-season rice growing regions of sub-counties and cities in Hubei province were investigated further. The potential yield of middle-season rice in Hubei province is great and the theoretical value can reach 11877957 tons. The main limiting factors of potential yield are the arable land and the climate. The results of this study and the established database will provide valuable referees for guidance and plan of rice cultivation, industrial restructuring and implementation of high technology project in Hubei province.