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光合細(xì)菌混合菌群利用牛糞污水產(chǎn)氫實(shí)驗(yàn)
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國家自然科學(xué)基金資助項(xiàng)目(51376056、U1404521)、教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金資助項(xiàng)目(20134105130001)和國家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)資助項(xiàng)目(2012AA051502)


Hydrogen Production Characteristics of Mixed Culture of Photosynthetic Bacteria Using Cattle Manure Wastewater
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    摘要:

    從自然界多處污泥中取樣,采用選擇性培養(yǎng)基初步富集出光合細(xì)菌混合菌群,采用菌體部分回流法對(duì)混合菌群進(jìn)行篩選和優(yōu)化,并研究了混合菌群以牛糞污水為原料的產(chǎn)氫特性。結(jié)果表明:從活性污泥中利用選擇性培養(yǎng)基富集出的光合細(xì)菌混合菌群,生長快速、穩(wěn)定,生長條件和產(chǎn)氫條件都比純種細(xì)菌要求低。采用菌體部分回流裝置篩選出了具有較高產(chǎn)氫活性的光合產(chǎn)氫混合菌群,菌體回流的最佳條件為:菌體回流時(shí)間36h,菌體回流量30%,此時(shí)混合菌群的產(chǎn)氫活性較高,最大產(chǎn)氫速率達(dá)到28.3mL/(L?h),平均氫氣體積分?jǐn)?shù)為55%。混合菌群以牛糞污水為原料產(chǎn)氫時(shí),產(chǎn)氫持續(xù)時(shí)間216h,平均產(chǎn)氫速率為11.65mL/(L?h),原料利用率為71.48%,平均原料轉(zhuǎn)化率為52.60mL/g。

    Abstract:

    For photo-hydrogen production offers the possibility of being renewable and carbon neutral, it has promising prospects in the clean energy future. The microorganisms from several natural sludge pools where has plenty of sunlight and with high organic load were used as samples. The mixed culture of photosynthetic bacteria (PSB) was cultivated by using selective medium method. Bacteria partial reflux device was employed for screening and optimization of mixed culture. The hydrogen production characteristics of mixed culture of PSB using cattle manure wastewater were also investigated. The results showed that, the mixed PSB culture grew fast and stable, and the conditions for its growth and hydrogen production were less strict than that of pure PSB. The optimum conditions for bacterial reflux were cell reflux time of 36h and cell reflux volume of 30%. The maximum hydrogen production rate was 28.3mL/(L·h), and mean concentration of H2 was 55%. When using cattle manure wastewater as substrate for biohydrogen production by mixed PSB, the hydrogen production duration was up to 216h, the average hydrogen production rate was 11.65mL/(L·h), substrate utilization ratio was 71.48%, and the average substrate conversion rate was 52.60mL/g. 

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王毅,張志萍,張全國,霍非,葉楊麗,于秀男.光合細(xì)菌混合菌群利用牛糞污水產(chǎn)氫實(shí)驗(yàn)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2015,46(5):214-219. Wang Yi, Zhang Zhiping, Zhang Quanguo, Huo Fei, Ye Yangli, Yu Xiunan. Hydrogen Production Characteristics of Mixed Culture of Photosynthetic Bacteria Using Cattle Manure Wastewater[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(5):214-219.

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  • 收稿日期:2014-08-19
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  • 在線發(fā)布日期: 2015-05-10
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