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乳鐵蛋白—多酚對(duì)β-胡蘿卜素乳液穩(wěn)定性的影響
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國(guó)家自然科學(xué)基金資助項(xiàng)目（31371835）


Effect of Lactoferrin-polyphenol Conjugates on Stability of β-carotene Emulsions
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    研究乳鐵蛋白（LF）—多酚（表沒(méi)食子兒茶素沒(méi)食子酸酯（EGCG）、綠原酸（CA）、沒(méi)食子酸（GA））共價(jià)復(fù)合物對(duì)β-胡蘿卜素乳狀液穩(wěn)定性的影響。采用自由基接枝聚合法制備乳鐵蛋白—多酚共價(jià)復(fù)合物并進(jìn)行高效液相色譜檢測(cè)。以乳鐵蛋白—多酚共價(jià)復(fù)合物為乳化劑制備β-胡蘿卜素乳狀液，通過(guò)加速分層和量化沉淀、懸浮的方法快速測(cè)定乳狀液的穩(wěn)定性；通過(guò)光照和不同溫度（25℃、37℃和55℃）條件下的貯藏實(shí)驗(yàn)，測(cè)定乳狀液中β-胡蘿卜素的化學(xué)穩(wěn)定性。結(jié)果表明，乳鐵蛋白經(jīng)多酚修飾后，色譜峰保留時(shí)間縮短，親水性增加。乳鐵蛋白與多酚生成的共價(jià)復(fù)合物可以提高乳狀液的穩(wěn)定性，同時(shí)可以抑制水包油乳狀液中β-胡蘿卜素的降解，提高β-胡蘿卜素的化學(xué)穩(wěn)定性。3種共價(jià)復(fù)合物中，LF—CA共價(jià)復(fù)合物對(duì)β-胡蘿卜素乳狀液具有最好的穩(wěn)定效果。

    Abstract:

    The objective of the study was to investigate the effects of lactoferrin-polyphenol conjugates on the stability of β-carotene emulsions. Conjugates of lactoferrin with polyphenol ((-)-epigallocatechin-3-gallate(EGCG), chlorogenic acid and gallic acid) were achieved by a free radical grafting procedure. The hydrophilic/hydrophobic characteristics of latoferrin and lactoferrin-polyphenol conjugates were studied by reverse-phase HPLC. Lactoferrin-polyphenol conjugates were used as emulsifiers to prepare β-carotene emulsions, and the stability of β-carotene emulsions was characterized by transmission profiles through using novel centrifugal sedimentation and β-carotene degradation in the emulsion during storage. The results implied that hydrophilicity of lactoferrin was increased after modified by polyphenol; compared with those stabilized by lactoferrin alone, the emulsions stabilized by lactoferrin-polyphenol conjugates were able to improve the stability of β-carotene emulsions and inhibit the deterioration of β-carotene in oil-in-water emulsions, and lactoferrin-chlorogenic acid conjugate was the best emulsifier among the lactoferrin-EGCG, lactoferrin-chlorogenic acid and lactoferrin-gallic acid conjugates. 

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劉夫國(guó),王迪,楊偉,袁芳,高彥祥.乳鐵蛋白—多酚對(duì)β-胡蘿卜素乳液穩(wěn)定性的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2015,46(6):212-217,225. Liu Fuguo, Wang Di, Yang Wei, Yuan Fang, Gao Yanxiang. Effect of Lactoferrin-polyphenol Conjugates on Stability of β-carotene Emulsions[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(6):212-217,225.

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  • 收稿日期:2014-08-05
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  • 在線(xiàn)發(fā)布日期: 2015-06-10
  • 出版日期: 2015-06-10