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Both-root-cut grafting is a new method in seedlings grafting, which can result in a greater production in a longer harvesting period. In order to improve the automation level of grafting process, especially the current situation of grafted seedlings relying on manual carrying and artificial replanting, a mechanism of automatically carrying and replanting both-root-cut grafted seedlings was developed. The working principle of newly designed handling and replanting was introduced. The simulation and kinematic analysis of key mechanisms were carried out. Experiments of grafting seedling area statistics and automatic carrying seedlings were conducted in laboratory, the results showed that the success rate of carrying seedlings was up to 94% and the average time of carrying was 2.5s, while the clamping mechanism was located at the lower end of the clip. Pressing experiment of three levels and three factors was designed to deal with the following conditions: the drilling hole diameter, the depth of punch and the suppression scheme of the grafted seedlings. While the hole diameter, the depth of drilling, and the pressing block was 10mm, 15mm, and a cylinder with lower surface separately, the effect of experiments was the best. Under this condition, the comprehensive success rate of grafted seedlings was up to 92%, and the average time of replanting was 2.5s. The experimental results showed that the automatic carrying device and the replanting device can be well matched with the grafting machine. And they can improve the automation level of the grafting process.