Abstract:The aim was to predict the growth of specific spoilage organisms (SSO) for Lateolabrax japonicus at different storage temperatures. The circulation of Lateolabrax japonicus was simulated by different storage methods, such as slurry ice storage (-1.8℃), crush ice storage (0℃), cold storage 1 (4℃) and cold storage 2 (10℃). The kinetic growth of total viable counts, Shewanella counts and Pseudomonas counts were analyzed respectively, and the total volatile base nitrogen (TVB-N) and Quality index (QI) value at the end of the shelf life of Lateolabrax japonicus were evaluated at different temperatures. On the basis of the dynamic change of SSO with modified Gompertz equation, Belehradek equations and Arrhenius equations were used to construct the prediction models of growth and shelf life for SSO. The results showed that the modified Gompertz equation could describe the dynamic microbial growth in Lateolabrax japonicus at different storage temperatures and the average minimum corruption values of Shewanella counts and Pseudomonas counts were (6.48±0.41) lg CFU/g, (6.33±0.36) lg CFU/g respectively. Meanwhile, the applicability of models were validated under crush ice storage (0℃) and temperature-fluctuation conditions (slurry ice precooling (-1.8℃)-transportation without ice (0.8℃) -crush ice (0℃)). The bias factors (Bf) and accuracy factors (Af) of Shewanella counts and Pseudomonas counts from the models based on Belehradek equations were 0.9936, 0.9510 and 1.0242, 0.9821 and 1.0845, 1.0425, 1.1075, 1.0934, respectively, and the relative errors of shelf life model were within the range of 0~10%. The accuracy of Belehradek equation was better than that of Arrhenius equation. In conclusion, the models obtained by Belehradek equations were more effective for predicting the growth of SSO and the shelf life of Lateolabrax japonicus at different storage temperatures so as to provide the theoretical basis for predicting the shelf life of Lateolabrax japonicus.
