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宰后藏羊肉Hsp70表達(dá)量變化及其與肉品質(zhì)相關(guān)性分析
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國(guó)家自然科學(xué)基金項(xiàng)目(31460433)和河西學(xué)院科研創(chuàng)新與應(yīng)用校長(zhǎng)基金項(xiàng)目(XZ2016007)


Correlation Analysis between Heat Shock Protein 70 Expression and Eating Quality during Postmortem Aging of Oula Tibetan Sheep Meat
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    摘要:

    為研究歐拉藏羊肉宰后成熟過(guò)程背最長(zhǎng)肌中熱休克蛋白Hsp70表達(dá)量的變化及其與肉品質(zhì)間的關(guān)系,選取了24頭甘南歐拉藏母羊,宰后在4℃環(huán)境中成熟,在成熟過(guò)程的不同時(shí)間點(diǎn)(2、12、24、48、72、120、168h)進(jìn)行pH值、L*、a*、b*、剪切力、系水率、肌原纖維小片化指數(shù)(MFI)、糖原含量、Hsp70表達(dá)量的測(cè)定,并進(jìn)行Hsp70表達(dá)量與肉品質(zhì)指標(biāo)的相關(guān)性分析。結(jié)果表明:Hsp70表達(dá)量與MFI呈極顯著負(fù)相關(guān)(P<0.01),與L*呈顯著負(fù)相關(guān)(P<0.05),與剪切力、糖原含量呈顯著正相關(guān)(P<0.05),與系水率呈極顯著正相關(guān)(P<0.01)。

    Abstract:

    The 70kDa heat shock protein (Hsp70) is a molecular chaperone that participates in various cellular processes, the role and significance of Hsp70 in postmortem muscle still remains unclear. The objective was to evaluate the relationship between the expression of heat shock protein 70 (Hsp70) and eating quality of Oula Tibetansheep meat during postmortem aging. Totally 24 female Oula Tibetan sheep in Gannan were slaughtered at 4℃ to aging,and then the pH value, chromaticity, shear force, WHC, MFI, glycogen and Hsp70 concentration were determined during postmortem aging, and the relationship between Hsp70 and eating quality was determined. Statistical analysis indicated that Hsp70 level had significant negative correlation with MFI (P<0.01) and L*(P<0.05), while it was significantly and positively correlated with shear force, glycogen(P<0.05) and WHC (P<0.01), respectively. The results suggested that Hsp70 may be involved in postmortem meat quality development. The research result can provide an important theoretical basis for the improvement of the quality of the Tibetan sheep meat during postmortem.

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張愛(ài)萍,師希雄,韓玲,岳建偉,孫金龍,張有富.宰后藏羊肉Hsp70表達(dá)量變化及其與肉品質(zhì)相關(guān)性分析[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2018,49(7):375-381. ZHANG Aiping, SHI Xixiong, HAN Ling, YUE Jianwei, SUN Jinlong, ZHANG Youfu. Correlation Analysis between Heat Shock Protein 70 Expression and Eating Quality during Postmortem Aging of Oula Tibetan Sheep Meat[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(7):375-381.

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  • 收稿日期:2018-01-04
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  • 在線發(fā)布日期: 2018-07-10
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