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微酸性電解水對灰葡萄孢菌殺菌效果與作用機(jī)制研究
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農(nóng)業(yè)農(nóng)村部設(shè)施農(nóng)業(yè)裝備與信息化重點(diǎn)實(shí)驗(yàn)室開放基金項(xiàng)目(NYBLAB-ZJU-2015-1)和煙臺市科技發(fā)展計(jì)劃項(xiàng)目(2012130)


Investigation on Microbicidal Potential and Action Mechanism for Botrytis cinerea of Slightly Acidic Electrolyzed Water
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    研究了微酸性電解水對灰葡萄孢菌懸液純培養(yǎng)及原位培養(yǎng)的殺菌效果,同時(shí)通過掃描電鏡和透射電鏡初步探究了其對灰葡萄孢菌的殺菌機(jī)制。研究表明,隨殺菌時(shí)間和有效氯質(zhì)量濃度的增加,微酸性電解水對灰葡萄孢的殺菌能力明顯增強(qiáng);在有效氯質(zhì)量濃度為10mg/L、殺菌處理15min,以及有效氯質(zhì)量濃度在30mg/L、殺菌處理10min時(shí),對懸液中灰葡萄孢菌的殺菌率均達(dá)99.99%。對用微酸性電解水處理后的灰葡萄孢菌落原位生長狀態(tài)觀察表明,微酸性電解水可以抑制菌落擴(kuò)生,濃度越高抑生長作用越強(qiáng),用有效氯質(zhì)量濃度為30mg/L的微酸性電解水處理菌落3d后,菌絲逐漸變暗黃、枯萎,直至死亡,菌落停止生長,推測微酸性電解水可殺滅灰葡萄孢的新生孢子并抑制菌絲生長。通過掃描及透射電鏡觀察,發(fā)現(xiàn)微酸性電解水處理可導(dǎo)致灰葡萄孢孢子細(xì)胞質(zhì)壁分離,細(xì)胞質(zhì)溢出,細(xì)胞器溶解,細(xì)胞皺縮,這可能是引起灰葡萄孢菌無法正常生長繁殖,最終達(dá)到殺菌效果的原因。

    Abstract:

    Slightly acidic electrolyzed water is a kind of bactericidal preparation with pH value slightly lower than 7.0 by electrolyzing dilute hydrochloric acid solution or electrolyzing low concentration dilute hydrochloric acid and electrolyte (NaCl/KCl) under nondiaphragm condition. It has a strong ability to kill pathogenic bacteria, but there is little research on the ability and mechanism of killing fungal spores by slightly acidic electrolyzed water. The killing effect of slightly acidic electrolytic water on pure culture spore suspension and in situ culture of Botrytis cinerea was studied, and the germicidal mechanism of water on Botrytis cinerea was preliminarily studied by scanning electron microscopy and transmission electron microscopy. The results showed that the bactericidal ability of slightly acidic electrolyzed water to Botrytis cinerea was increased significantly with the increase of sterilization time and available chlorine concentration (ACC), and bactericidal rate can achieve 99.99% when the available chlorine concentration was not less than 30mg/L and kill time was 10min. The growth state of Botrytis cinerea colony in situ after treatment with slightly acidic electrolytic water showed that slightly acidic electrolytic water could inhibit colony expansion, and the higher the concentration was, the stronger the inhibition effect was. After 3d of treatment with slightly acidic electrolytic water with 30mg/L available chlorine, the mycelium gradually turned yellow and withered until death, and the colony was stopped. It was suggested that microacid electrolyzed water can kill the new spores of Botrytis cinerea and inhibit the growth of mycelia. Through microscope observation of scanning and transmission electron, it was found that treatment with acidic electrolyzed water can make separation of membrane and cell wall, cytoplasmic overflow and abnormal lysis of organelles. It was speculated that these may be the reasons which can achieve the bactericidal effect.

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南松劍,黃曉伶,王朔,葉章穎,朱松明.微酸性電解水對灰葡萄孢菌殺菌效果與作用機(jī)制研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2019,50(1):354-358. NAN Songjian, HUANG Xiaoling, WANG Shuo, YE Zhangying, ZHU Songming. Investigation on Microbicidal Potential and Action Mechanism for Botrytis cinerea of Slightly Acidic Electrolyzed Water[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(1):354-358.

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  • 收稿日期:2018-10-04
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  • 在線發(fā)布日期: 2019-01-10
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