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Aiming at the intelligent development of agricultural machinery and equipment at home and abroad and the remote network control requirements of equipment and materials, based on ISO 11783 agricultural machine CAN bus standard, the intelligent harvesting CAN bus program and its application system were proposed and designed. According to the operation characteristics and intelligent control requirements of the combine harvester, a modular and expandable intelligent agricultural CAN bus network structure, consisting of a power CAN bus, a device management CAN bus, a dedicated device CAN bus 1 and a dedicated device CAN bus 2 was established. Based on the ISO 11783 CAN bus application layer protocol standard, an intelligent remote harvester remote network control communication protocol was developed. Finally, the network load and real-time communication experiment of CAN bus application system of intelligent combine harvester and the engineering application experiment were carried out. The results showed that at the baud rate of 500kb/s, the load of all buses was less than 30%, and the data transmission delay between the three layers CAN buses was less than 1ms, which fully met the design requirements of the intelligent remote network-controlled CAN bus system of combine harvester. In engineering applications, it also met the automatic navigation communication requirements of harvesters.