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基于指紋圖譜的歐拉羊肉揮發(fā)性風味物質(zhì)定量分析
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國家級大學生創(chuàng)新創(chuàng)業(yè)訓練計劃項目(201910019084)和國家肉羊產(chǎn)業(yè)技術(shù)體系項目(CARS-38)


Quantitative Analysis of Oula Lamb Meat Volatile Compounds Based on Fingerprint
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    摘要:

    歐拉羊分布在青藏高原東部,生活環(huán)境特殊,羊肉品質(zhì)獨特,但其揮發(fā)性風味物質(zhì)組成尚不明確。為研究歐拉羊肉熟制時的揮發(fā)性風味物質(zhì),并構(gòu)建相應指紋圖譜,以歐拉羊、湖羊、杜泊羊和灘羊羊肉為研究對象,利用頂空固相微萃取、結(jié)合氣相色譜質(zhì)譜聯(lián)用法,對羊肉揮發(fā)性風味物質(zhì)進行定性定量分析,并通過嗅辨儀鑒定出呈香化合物。運用主成分分析和聚類分析,將歐拉羊與湖羊、杜泊羊、灘羊羊肉的風味進行比較,從而明確歐拉羊肉的揮發(fā)性風味物質(zhì),并建立指紋圖譜。結(jié)果表明,在歐拉羊肉中檢測到醛類、酮類、醇類、雜環(huán)類、烷烴類等共53種揮發(fā)性物質(zhì),其中77%具有氣味活性。所有物質(zhì)中醛類有20種,在整體風味物質(zhì)中的相對含量占比達62%,對風味貢獻最大。歐拉羊肉整體風味物質(zhì)輪廓與其他3種羊肉有顯著區(qū)別,其特征性風味物質(zhì)主要包括(E)-2-己烯醛、(E,E)2,4-庚二烯醛、(E,E)-2,4-辛二烯醛、3-辛烯-2-酮等不飽和醛酮,4-異丙基甲苯等烷烴類,以及苯并噻唑等其他呈味物質(zhì),它們共同貢獻了水果香、堅果味、青草味等令人愉悅的氣味。

    Abstract:

    Distributed in the eastern Qinghai-Tibet plateau where the environmental condition is unique, Oula lamb is of distinction owing to the quality of its meat. Aiming to establish the fingerprint of volatile flavor for Oula lamb meat. The lamb meat was obtained from four kinds of lamb: Oula lamb, Hu lamb, Dorper lamb and Tan lamb. Extraction and analysis of the volatile compounds were carried out by headspace solidphase microextraction and gas chromatography-mass spectrometry respectively. At the same time, gas chromatography-olfactometry was used to assess the fragrant compounds. Principal component analysis and cluster analysis were applied to determine the chemical variability among four kinds of lamb meat. The results showed that 53 volatile components of Oula lamb meat were identified and quantified, including aldehydes, heterocycles, hydrocarbons, alcohols and ketones, among which 77% possessed perceptible aromas. Totally 20 kinds of aldehydes with their content accounting for 62% contributed the most to flavor. Grouping of samples highlighted a good classification of the results, providing a significant separation between Oula lamb meat and other three kinds of lamb meat. Characteristic flavor compounds of Oula lamb meat mainly included (E)-2-hexenal, (E,E)-2,4-heptadienal, (E,E)-2,4-octadienal, 3-octen-2-one and other unsaturated aldehydes and ketones, together with some hydrocarbons, such as p-cymene, and other flavor compounds such as benzothiazole, providing fruits, nuts and grass odors. The findings provided the volatile fingerprint of Oula lamb meat, which can promote the development of related products of Oula lamb.

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陳學敏,朱國茵,羅海玲,余忠祥,羅潔,張昊.基于指紋圖譜的歐拉羊肉揮發(fā)性風味物質(zhì)定量分析[J].農(nóng)業(yè)機械學報,2020,51(5):349-355. CHEN Xuemin, ZHU Guoyin, LUO Hailing, YU Zhongxiang, LUO Jie, ZHANG Hao. Quantitative Analysis of Oula Lamb Meat Volatile Compounds Based on Fingerprint[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(5):349-355.

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  • 收稿日期:2020-01-23
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  • 在線發(fā)布日期: 2020-05-10
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