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添加菊粉條件下的豌豆分離蛋白乳化特性研究
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黑龍江省自然科學(xué)基金聯(lián)合引導(dǎo)項(xiàng)目(LH2019C032)


Emulsifying Properties of Pea Protein Isolate with Inulin
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    摘要:

    為探究添加不同質(zhì)量分?jǐn)?shù)(1%、3%、5%和7%)菊粉(Inulin, INU)對(duì)豌豆分離蛋白(Pea protein isolate, PPI)乳化性及乳液穩(wěn)定性的影響,以PPI作為乳化劑,采用高壓均質(zhì)法制備了PPI/INU乳液,通過(guò)zeta電位測(cè)定、粒徑測(cè)定、激光共聚焦顯微鏡(CLSM)、酶標(biāo)法和內(nèi)源熒光光譜等技術(shù)對(duì)乳液進(jìn)行表征。結(jié)果表明:添加1%INU后,乳液具有最大zeta電位絕對(duì)值(為34.03mV)和最小平均粒徑(d4,3為395.50nm);CLSM顯示,低濃度(質(zhì)量分?jǐn)?shù)1%和3%)的INU使乳液液滴分布更均勻;INU質(zhì)量分?jǐn)?shù)為1%時(shí),分別使PPI的乳化活性指數(shù)、乳化穩(wěn)定性指數(shù)和乳液界面蛋白吸附率增加了7.8%、22%和11%;熒光光譜顯示,隨著INU濃度的增加,連續(xù)相中PPI-INU復(fù)合物的生成量增多,對(duì)乳液的穩(wěn)定性產(chǎn)生了負(fù)面影響。由此說(shuō)明低濃度(質(zhì)量分?jǐn)?shù)為1%和3%)的INU可改善PPI的乳化性、提高PPI乳液的穩(wěn)定性,其中添加1%INU效果最顯著。

    Abstract:

    Aiming to investigate the effects of different inulin (INU) concentrations (1%, 3%, 5% and 7%) on the emulsification of pea protein isolate (PPI) and its emulsion stability, and determine the optimal concentration of INU. PPI/INU emulsion was prepared by high pressure homogenization method using PPI as emulsifier. The emulsion was characterized by particle size, zeta potential, laser confocal microscopy (CLSM), enzyme labeling and endogenous fluorescence spectroscopy. The results showed that low concentrations (1% and 3%) of INU had a beneficial effect on zeta potential and average particle size, with an absolute maximum zeta potential at 1% INU (34.03mV) and a minimum mean particle size (d4,3 was 395.50nm). CLSM showed more uniform droplet distribution in emulsions with low concentrations (1% and 3%). When the concentration of INU was 1%, the emulsification activity index and emulsion stability index of PPI were increased by 7.8% and 22%, respectively, emulsion interface protein adsorption rate was increased by 11%. The fluorescence spectrum showed that with the increase of concentration of INU, the amount of PPI-INU complexes in the continuous phase was increased, which had a negative impact on the stability of the emulsion. It was concluded that low concentrations (1% and 3%) of INU could significantly improve the emulsification of PPI and enhance the stability of PPI emulsion, with 1% of INU being the most significant.

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李楊,徐清清,韓璐,王迪瓊,齊寶坤.添加菊粉條件下的豌豆分離蛋白乳化特性研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2020,51(7):382-388. LI Yang, XU Qingqing, HAN Lu, WANG Diqiong, QI Baokun. Emulsifying Properties of Pea Protein Isolate with Inulin[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(7):382-388.

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  • 收稿日期:2019-09-15
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  • 在線發(fā)布日期: 2020-07-10
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