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基于SPME-GC-MS和電子鼻的魚(yú)粉揮發(fā)性物質(zhì)分析
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中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金項(xiàng)目(2662018PY081、11041910103)


Analysis of Volatile Compounds in Fish Meal Based on SPME-GC-MS and Electronic Nose
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    摘要:

    為了能夠全面、快速分析魚(yú)粉的新鮮度,將電子鼻與頂空固相微萃取氣質(zhì)聯(lián)用技術(shù)結(jié)合,以揮發(fā)性物質(zhì)含量輔助評(píng)價(jià)魚(yú)粉的新鮮度。首先,采用頂空固相微萃取方法對(duì)18種儲(chǔ)藏時(shí)間魚(yú)粉的揮發(fā)性物質(zhì)進(jìn)行了分析,得到不同儲(chǔ)藏時(shí)間魚(yú)粉的揮發(fā)性物質(zhì)種類(lèi)和含量,以及揮發(fā)性物質(zhì)變化規(guī)律;采用多元線性回歸方法和最近鄰回歸方法對(duì)魚(yú)粉各類(lèi)揮發(fā)性物質(zhì)和表征新鮮度的標(biāo)記物與電子鼻傳感器響應(yīng)建立了模型。結(jié)果表明:頂空固相微萃取氣質(zhì)聯(lián)用(Solid phase microextraction-gas chromatography-mass spectrometry, SPME-GC-MS)共鑒定出101種,主要包括醇類(lèi)化合物、醛類(lèi)化合物等11類(lèi)揮發(fā)性物質(zhì),其中醛酮類(lèi)化合物種類(lèi)較多。隨著魚(yú)粉逐漸腐敗,氣味閾值較低的醛類(lèi)化合物相對(duì)含量逐漸降低、酮類(lèi)化合物相對(duì)含量逐漸升高,并出現(xiàn)了二甲基三硫、三甲胺等表征魚(yú)粉新鮮度的標(biāo)記物;在儲(chǔ)藏過(guò)程中,醇類(lèi)化合物相對(duì)含量呈先上升后下降趨勢(shì);酸類(lèi)化合物、含氮化合物、含硫化合物相對(duì)含量則呈上升趨勢(shì)。與多元線性回歸方法相比,最近鄰回歸方法具有更好的預(yù)測(cè)能力,測(cè)試集相關(guān)系數(shù)在0.7633~0.9999之間,均方根誤差在0.0867%~8.4655%之間。

    Abstract:

    In order to analyze the freshness of fish meal comprehensively and quickly, the electronic nose and SPME-GC-MS were combined to evaluate the freshness of fish meal through the content of volatile components. The volatile components of fish meal with 18 different storage times levels was analyzed by solid-phase headspace microextraction, and the kinds and contents of volatile components in fish meal were obtained at different storage time, and the change rule of volatile components was obtained. Multiple linear regression and KNN regression were used to model various volatile components and freshness markers of fish meal and the response of electronic nose sensors. A total of 101 volatile compounds were detected, including 11 alcohols, 20 aldehydes, 26 ketones, 8 acids, 9 hydrocarbons, 15 nitrogen-containing compounds, 4 sulfur-containing compounds and other compounds. Among them, there were more aldehyde ketones, less esters, phenols and ethers. Ethoxyquin and γ-dehydrogenase were also detected in the volatile gas of fish meal, the reason was that fish meal, as a feed material, was not only a simple processing of raw fish, and also required other additives to facilitate the transportation and storage of fish meal. At the beginning of storage, the relative content of aldehydes was 48.99%, but at the end of storage, the relative content of aldehydes was 0.95%. The relative content of aldehydes was significantly reduced, while the relative content of ketones was increased. At the beginning of storage, the relative content of ketones was 28.55%, but at the end of storage, it was 51.43 %. The results showed that the freshness of fish meal was characterized by trimethylamine, 3-methyl-1-butanol, dimethyl disulfide and dimethyl trisulfide. The relative contents of alcohols showed a downward trend after the first increase, acids, nitrogenous compounds and sulfur compounds showed an upward trend. The models of various volatile components in fish meal and the markers representing freshness and the sensor responses of electronic nose were established by multiple linear regression method and KNN regression method. The results showed that the KNN regression method had higher accuracy than multiple linear regression method. The correlation coefficient was between 0.7633 and 0.9999, and RMSE was between 0.0867% and 8.4655%.Therefore, these volatile components can be well predicted from electronic nose measurement. The results can be used as reference for understanding the flavor composition and change rule of volatile components of fish meal during storage, and for judging the freshness of fish meal according to the smell component.

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李培,牛智有,朱明,邵愷懌,耿婕,李洪成.基于SPME-GC-MS和電子鼻的魚(yú)粉揮發(fā)性物質(zhì)分析[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2020,51(7):397-405. LI Pei, NIU Zhiyou, ZHU Ming, SHAO Kaiyi, GENG Jie, LI Hongcheng. Analysis of Volatile Compounds in Fish Meal Based on SPME-GC-MS and Electronic Nose[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(7):397-405.

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  • 收稿日期:2020-04-01
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  • 在線發(fā)布日期: 2020-07-10
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