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牛奶中孕酮的金鐵復(fù)合納米粒子磁弛豫開關(guān)傳感檢測(cè)
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國家自然科學(xué)基金項(xiàng)目(81773482)


Magnetic Relaxation Switch Sensor for Rapid Detection of Progesterone in Milk Based on Aptamers Functionalized Au@Fe3O4 Nanoparticles
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    摘要:

    孕酮是由黃體分泌的一種類固醇激素,食品中存在的孕酮對(duì)人體內(nèi)分泌等健康產(chǎn)生嚴(yán)重影響。因此,探索簡(jiǎn)單、快速、靈敏的孕酮檢測(cè)技術(shù)及方法很有必要。基于孕酮的特異性適配體(Apt)功能化的Au@Fe3O4納米粒子構(gòu)建磁弛豫傳感器,在優(yōu)化各種實(shí)驗(yàn)條件的基礎(chǔ)上,對(duì)傳感器的靈敏度、特異性進(jìn)行評(píng)估,并對(duì)牛奶中孕酮檢測(cè)的可行性進(jìn)行分析。當(dāng)孕酮適配體與Au@Fe3O4納米粒子的體積比為3且孵育1.5h時(shí),有利于Apt-Au@Fe3O4納米探針的構(gòu)建。磁弛豫檢測(cè)過程中的優(yōu)化條件如下:NaCl濃度為0.02mol/L,Apt-Au@Fe3O4納米粒子稀釋400倍,反應(yīng)15min。在此條件下,水體系中單組份橫向弛豫時(shí)間的變化量與孕酮濃度間呈良好的線性關(guān)系,線性區(qū)間為1~40nmol/L,檢測(cè)限為4.97nmol/L,且具有良好的特異性。該方法可實(shí)現(xiàn)對(duì)牛奶樣品中孕酮的檢測(cè),對(duì)牛奶中孕酮的線性檢測(cè)區(qū)間為20~100nmol/L,檢測(cè)限為13.60nmol/L,加標(biāo)回收率為99.21%~103.45%,相對(duì)標(biāo)準(zhǔn)偏差不大于9.02%。

    Abstract:

    With the increase of public attention on food safety, it is important to establish a sensitive and rapid detection method for progesterone (pregn-4-ene-3,20-dione, P4) in animal food products. A magnetic relaxation switch (MRS) sensor based on aptamer functionalized Au@Fe3O4 nanoparticles (NPs) was developed for the detection of progesterone in milk. The test conditions were optimized to improve the sensitivity and stability of the method, and the feasibility of this method for the detection of progesterone in milk was also analyzed.The ratio of aptamers (Apt) to Au@Fe3O4 NPs, the incubation time of aptamers modification, the concentration of NaCl, the dilute ratio of Apt-Au@Fe3O4 and reaction time were optimized to be 3, 1.5 h, 0.02 mol/L, 400 and 15min, respectively. Under the optimized conditions, the change of mono-component relaxation time (ΔT2w) showed a linear range from 1nmol/L to 40nmol/L and the limitation of detection (LOD) was 4.97 nmol/L. In addition, the proposed method also illustrated an excellent specificity. The method was also applied successfully for the detection of progesterone in milk samples. The MRS displayed linear ranges of detecting progesterone from 20 nmol/L to 100 nmol/L and LOD of 13.60 nmol/L in milk. The average recoveries of progesterone in milk were 99.21%~103.45%, with relative standard deviations of nomore than 9.02%. The results indicated that this sensor could be a potential tool for the rapid detection of progesterone in food.

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王欣,王琪,孫涵穎,裴凱麗.牛奶中孕酮的金鐵復(fù)合納米粒子磁弛豫開關(guān)傳感檢測(cè)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2022,53(4):412-422. WANG Xin, WANG Qi, SUN Hanying, PEI Kaili. Magnetic Relaxation Switch Sensor for Rapid Detection of Progesterone in Milk Based on Aptamers Functionalized Au@Fe3O4 Nanoparticles[J]. Transactions of the Chinese Society for Agricultural Machinery,2022,53(4):412-422.

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  • 收稿日期:2021-04-08
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  • 在線發(fā)布日期: 2021-05-27
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