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Photosynthesis directly affects the quality and yield of blueberries, and the photosynthetic rate of crops is mainly affected by temperature, light and CO2. At present, the control of light and CO2 is mainly based on the saturation point of photosynthesis rate, and the control and control efficiency is low. Aiming at the current problems of greenhouse light and CO2 control efficiency, a comprehensive control strategy of blueberry greenhouse light and CO2 based on the maximization of Gaussian curvature was proposed. Firstly, by collecting the blueberry net photosynthetic rate under different temperatures, light, and CO2 nesting, a blueberry net photosynthetic rate mechanism model was established, including light intensity and CO2 concentration at different temperatures;then according to the Gaussian curvature function of the blueberry photosynthetic rate mechanism model at different temperatures, the fitness function was constructed, and the particle swarm algorithm was used to optimize the maximum value, the light intensity and CO2 concentration corresponding to the maximum Gaussian curvature were calculated, and the maximum point of light and CO2 Gaussian curvature at different temperatures were obtained;finally, based on polynomial fitting, different comprehensive control strategy of light and CO2 was established at different temperatures. By comparing with the control of the saturation point of the maximum net photosynthetic rate, it was found that the average light intensity was decreased by 60.73%, the CO2 concentration was decreased by 25.00%, and the average net photosynthetic rate was only decreased by 14.29%. Compared with the actual blueberry net photosynthetic rate, it was found that using the proposed comprehensive control strategy of light and CO2, the blueberry net photosynthetic rate was increased by more than 1.87 times on average compared with the actual value. It showed that the proposed comprehensive regulation strategy of illumination and CO2 had the characteristics of high comprehensive benefit, and it can provide theoretical support for the regulation of illumination and CO2 in greenhouse.