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In order to improve the transplanting efficiency of transplanting machine to adapt to the greenhouse, a double row of transplanting manipulator linkage type high-speed transplanting device was designed. Using the double row transplanting manipulator installed on two transplanting arms to alternately take and plant seedlings, the control mode can double the efficiency of transplanting. Through the analysis of transplanting work process, the transplanting arm and transplanting manipulators were optimized, and the key components were parametrically designed and kinematically analyzed. Finally, considering the key factors influencing the success rate of transplanting, and determining tray seedling moisture content, age of seedling and center distance of limit hole of claw needle as the experiment factors. Selecting 200 holes carnation hole tray seedling as experiment object, the single factor experiment and multifactor orthogonal experiment were carried out to explore the double row transplanting manipulator linkage type optimal working parameters of the high-speed transplanting mechanism. The experimental results showed that when the water content of the pot seedling was 32.6%, the seedling age was 46d, and the distance between the center of the limit hole of the paw needle was 16.7mm, the average value of success rate was of 94.7%, which was close to the software calculation result of 94.2%. The results could lay a foundation for the development of high-speed automatic transplanter for potted flowers which met the technical requirements of the transplanting operation of flower transplanter.