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不同多糖條件下大豆蛋白-綠原酸凝膠特性研究
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農(nóng)業(yè)農(nóng)村部大豆產(chǎn)業(yè)技術(shù)體系崗位科學(xué)家項(xiàng)目(CARS-04-PS32)


Characterization of Soy Protein-Chlorogenic Acid Gels under Different Polysaccharide Conditions
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    摘要:

    為研究添加果膠、黃原膠和κ-卡拉膠3種多糖的種類和質(zhì)量濃度(0.002、0.004、0.006g/mL)對(duì)大豆蛋白(SPI)-綠原酸(CA)水凝膠的凝膠特性和分子間作用力的影響,通過測(cè)定SPI-CA/多糖三元凝膠的紅外光譜,分子間作用力、流變學(xué)、水分分布和微觀結(jié)構(gòu)等指標(biāo),最終探明不同多糖與SPI-CA相互作用機(jī)制以及不同多糖對(duì)SPI-CA凝膠性質(zhì)改善的差異性和相似性。結(jié)果表明,添加黃原膠和κ-卡拉膠后,蛋白中β-折疊相對(duì)含量從24.59%升高至24.87%~26.65%,無規(guī)則卷曲含量顯著降低(P<0.05),這有利于凝膠網(wǎng)絡(luò)結(jié)構(gòu)的形成。3種多糖的添加均顯著增強(qiáng)了凝膠中氫鍵的作用以及凝膠中固定水的結(jié)合能力,相較于果膠和黃原膠,添加κ-卡拉膠后凝膠中的氫鍵的作用更強(qiáng),T21的弛豫時(shí)間更短,凝膠結(jié)構(gòu)也更加致密。凝膠的儲(chǔ)能模量G′、損耗模量G″、硬度和咀嚼性均與κ-卡拉膠的濃度呈正相關(guān)。適量的果膠和黃原膠的加入能夠形成穩(wěn)定的凝膠,但是過度填充果膠和黃原膠會(huì)降低凝膠的熱穩(wěn)定性,破壞凝膠網(wǎng)絡(luò)結(jié)構(gòu)。

    Abstract:

    With the aim to investigate the effects of adding three polysaccharide types and concentrations of pectin, xanthan gum and κ-carrageenan (0.002g/mL,0.004g/mL,0.006g/mL) on the gel properties and molecular forces of soy protein (SPI)-chlorogenic acid (CA) hydrogels, the infrared spectra, molecular forces, rheology, water distribution and microstructure of SPI-CA/polysaccharide ternary gels were measured. Finally, the interaction mechanism between different polysaccharides and SPI-CA and the differences and similarities of different polysaccharides on the improvement of SPI-CA gel properties were confirmed. The results showed that the addition of xanthan gum and κ-carrageenan increased the β-sheets content of the protein from 24.59% to 24.87%~26.65% and significantly decreased the content of random coil (P<0.05), which was beneficial to the formation of the gel network structure. The addition of all three polysaccharides significantly enhanced the hydrogen bonding interactions in the gels and the binding ability of immobilized water (T21) in the gels. Compared with pectin and xanthan gum, the addition of κ-carrageenan resulted in stronger hydrogen bonding interactions in the gels, shorter relaxation time of T21, and denser gel structure. The storage modulus (G′), loss modulus (G″), hardness and chewiness of the gels were positively correlated with the concentration of κ-carrageenan. The addition of appropriate amounts of pectin and xanthan gum can form stable gels, but overfilling with pectin and xanthan gum can reduce the thermal stability of the gels and destroy the gel network structure. The results can provide theoretical support for introducing different polysaccharides to improve the gel properties of soy protein-chlorogenic acid hydrogels. 

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李楊,滿慧,賈亦佳,閆馨月,閆世長,IGOR A N.不同多糖條件下大豆蛋白-綠原酸凝膠特性研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2023,54(5):387-395. LI Yang, MAN Hui, JIA Yijia, YAN Xinyue, YAN Shizhang, IGOR A N. Characterization of Soy Protein-Chlorogenic Acid Gels under Different Polysaccharide Conditions[J]. Transactions of the Chinese Society for Agricultural Machinery,2023,54(5):387-395.

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  • 收稿日期:2022-08-29
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  • 在線發(fā)布日期: 2023-05-10
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